vectashield antifade mounting medium with dapi (Vector Laboratories)
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Vector Laboratories
vectashield antifade mounting medium with dapi
Vectashield Antifade Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 21989 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectashield-dapi/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/custom%40h-1200%4042755103
Average 98 stars, based on 21989 article reviews
Vectashield Antifade Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 21989 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectashield-dapi/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/custom%40h-1200%4042755103
Average 98 stars, based on 21989 article reviews
vectashield antifade mounting medium with dapi - by Bioz Stars,
2026-10
98/100 stars
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Staining:Article Title: Dual-function oral nanotherapeutic mitigates sepsis-like multi-organ failure by targeting inflammatory and fibrotic pathways Article Snippet: .. Thereafter, sections were stained with a recombinant rabbit monoclonal antibody against Nlrp3 (invitrogen, Cat#: MA5-32255, RRID: AB_2809541 ), Casp1 p20 (cleaved Asp296) polyclonal antibody (invitrogen, Cat#: PA5-99390, RRID: AB_2818323 ), and Nek7 polyclonal antibody (invitrogen, Cat#: PA5-101861, RRID: AB_2851293 ) at 1:100 dilution in 1.5% horse serum in PBS, while NF-κB p65 was incubated with mouse monoclonal antibody (SantaCruz, Cat#: A-12 sc: 514451, RRID: AB_2891257 ) at 1:25 dilution overnight at 4 °C followed by secondary antibody [goat anti-rabbit IgG Alexa Flour-488 (Nlrp3 and Nek7) and 594 (Casp1 and NF-κB p65)] at 1:1000 dilution in PBS for 2 h. Sections were then washed 3 times with PBS and counterstained with Recombinant:Article Title: Dual-function oral nanotherapeutic mitigates sepsis-like multi-organ failure by targeting inflammatory and fibrotic pathways Article Snippet: .. Thereafter, sections were stained with a recombinant rabbit monoclonal antibody against Nlrp3 (invitrogen, Cat#: MA5-32255, RRID: AB_2809541 ), Casp1 p20 (cleaved Asp296) polyclonal antibody (invitrogen, Cat#: PA5-99390, RRID: AB_2818323 ), and Nek7 polyclonal antibody (invitrogen, Cat#: PA5-101861, RRID: AB_2851293 ) at 1:100 dilution in 1.5% horse serum in PBS, while NF-κB p65 was incubated with mouse monoclonal antibody (SantaCruz, Cat#: A-12 sc: 514451, RRID: AB_2891257 ) at 1:25 dilution overnight at 4 °C followed by secondary antibody [goat anti-rabbit IgG Alexa Flour-488 (Nlrp3 and Nek7) and 594 (Casp1 and NF-κB p65)] at 1:1000 dilution in PBS for 2 h. Sections were then washed 3 times with PBS and counterstained with Incubation:Article Title: Dual-function oral nanotherapeutic mitigates sepsis-like multi-organ failure by targeting inflammatory and fibrotic pathways Article Snippet: .. Thereafter, sections were stained with a recombinant rabbit monoclonal antibody against Nlrp3 (invitrogen, Cat#: MA5-32255, RRID: AB_2809541 ), Casp1 p20 (cleaved Asp296) polyclonal antibody (invitrogen, Cat#: PA5-99390, RRID: AB_2818323 ), and Nek7 polyclonal antibody (invitrogen, Cat#: PA5-101861, RRID: AB_2851293 ) at 1:100 dilution in 1.5% horse serum in PBS, while NF-κB p65 was incubated with mouse monoclonal antibody (SantaCruz, Cat#: A-12 sc: 514451, RRID: AB_2891257 ) at 1:25 dilution overnight at 4 °C followed by secondary antibody [goat anti-rabbit IgG Alexa Flour-488 (Nlrp3 and Nek7) and 594 (Casp1 and NF-κB p65)] at 1:1000 dilution in PBS for 2 h. Sections were then washed 3 times with PBS and counterstained with |
![Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei <t>(DAPI;</t> blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0342/pmc12810342/pmc12810342__gr3.jpg)